NEUROLOGY

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CRISPR Helps Find New Genetic Suspects Behind ALS/FTD

 

Disease-causing mutations in C9orf72, insert extra sequences of DNA into the gene, called hexanucleotide repeats, account for nearly 40% of inherited cases of amyotrophic lateral sclerosis (ALS) and 25% of inherited frontotemporal dementia (FTD) cases. These repeats produce potentially toxic RNA and protein molecules that kill neurons resulting in problems with movement and eventually paralysis for ALS patients and language and decision-making problems for FTD patients.

 

According to an article published in Nature Genetics (5 March 2018), a new study provides a roadmap for using CRISPR to investigate neurological disorders. For the study, the authors used the gene editing tool CRISPR-Cas9 to rapidly identify genes in the human genome that might modify the severity of ALS and FTD caused by mutations in a gene called C9orf72. The results of the search uncovered a new set of genes that may hasten neuron death during the disease.

 

For the study, the authors used CRISPR to disable each gene, one-by-one, in a line of human leukemia cells and then tested whether the cells would survive exposure to toxic proteins derived from the hexanucleotide repeats, called DPRs. Any disabled genes that caused cells to live longer or die faster than normal were considered suspects in DPR toxicity. The authors confirmed that genes that control the movement of molecules in and out of a cell’s nucleus may be partners. They also identified several new players, including genes that modify chromosomes and that help cells assemble proteins passing through a maze-like structure called the endoplasmic reticulum (ER). A second CRISPR search conducted on mouse brain cells confirmed the initial results. Disabling the top 200 genes identified in the leukemia cells helped neurons survive DPR exposure. Finally, further experiments highlighted the importance of the ER genes, especially one called TMX2. For example, the authors could cause neurons derived from the skin cells of ALS patients with C9orf72 to live longer than normal when they silenced the TMX2 gene, suggesting it could be exploited in designing novel therapies for ALS. Decreasing TMX2 in cells caused an increase in the production of “survival proteins” that the authors hypothesized protected the cells against DPR toxicity.

 

Previously such studies needed a few months to find candidate genes and could only be performed on yeast, worm, and fly genomes. With CRISPR, the authors needed just about two weeks to conduct a complete search of the human genome. The results suggest that this faster and more comprehensive approach may be used to rapidly identify genes that may be involved in other neurological disorders.

 

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